dpbs buffer Search Results


phosphate buffered saline  (Thermo Fisher)
99
Thermo Fisher phosphate buffered saline
Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dpbs buffer  (MedChemExpress)
93
MedChemExpress dpbs buffer
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dpbs Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphate buffered saline  (Valiant Co Ltd)
96
Valiant Co Ltd phosphate buffered saline
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Phosphate Buffered Saline, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphate buffered saline/product/Valiant Co Ltd
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phosphate buffered saline - by Bioz Stars, 2026-03
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dpbs  (Valiant Co Ltd)
93
Valiant Co Ltd dpbs
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dpbs, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dpbs/product/Valiant Co Ltd
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dpbs - by Bioz Stars, 2026-03
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dulbecco  (Lonza)
97
Lonza dulbecco
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x dulbecco  (Valiant Co Ltd)
93
Valiant Co Ltd 1x dulbecco
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
1x Dulbecco, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco’s phosphate buffered saline dpbs  (Quality Biological Inc)
90
Quality Biological Inc dulbecco’s phosphate buffered saline dpbs
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco’s Phosphate Buffered Saline Dpbs, supplied by Quality Biological Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dulbecco’s phosphate-buffered saline solution  (Biowest SAS)
90
Biowest SAS dulbecco’s phosphate-buffered saline solution
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco’s Phosphate Buffered Saline Solution, supplied by Biowest SAS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco’s phosphate-buffered saline solution/product/Biowest SAS
Average 90 stars, based on 1 article reviews
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dulbecco’s phosphate-buffered saline (dpbs  (PAN - Biotech)
90
PAN - Biotech dulbecco’s phosphate-buffered saline (dpbs
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco’s Phosphate Buffered Saline (Dpbs, supplied by PAN - Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dulbecco’s phosphate-buffered saline corning dpbs  (Corning Life Sciences)
90
Corning Life Sciences dulbecco’s phosphate-buffered saline corning dpbs
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco’s Phosphate Buffered Saline Corning Dpbs, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco’s phosphate-buffered saline corning dpbs/product/Corning Life Sciences
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dulbecco’s phosphate-buffered saline dpbs  (Welgene inc)
90
Welgene inc dulbecco’s phosphate-buffered saline dpbs
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco’s Phosphate Buffered Saline Dpbs, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco’s phosphate-buffered saline dpbs/product/Welgene inc
Average 90 stars, based on 1 article reviews
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dulbecco's phosphate-buffered saline (dpbs  (Becton Dickinson)
90
Becton Dickinson dulbecco's phosphate-buffered saline (dpbs
Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with <t>DPBS</t> or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) <t>Relative</t> <t>NAD+</t> (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.
Dulbecco's Phosphate Buffered Saline (Dpbs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with DPBS or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) Relative NAD+ (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Stepwise activation of SARM1 for cell death and axon degeneration revealed by a biosynthetic NMN mimic.

doi: 10.1073/pnas.2424906122

Figure Lengend Snippet: Fig. 5. Introducing a disulfide bond between the ARM and SAM domains inhibited M1 or NMN- mediated SARM1 activation. (A) Cell survival rates in HeLa-SARM1−/− cells (Vector) and HeLa-SARM1−/− cells ectopically expressing SARM1 or SARM1 cysteine mutants, treated with 10 μM G10 for 24 h, determined by measuring ATP levels. Data are represented as mean ± SD from triplicate wells. (B) Time course of the base exchange activities of the purified SARM1 (WT) or SARM1 (2Cmut) incubated with DPBS or M1. Each bar represents mean ± SD, n = 3. ****P < 0.0001, n.s., not significant. (C and D) Relative NAD+ (C) and cADPR (D) levels in the reaction mixture containing purified SARM1 WT (Top) or 2Cmut (Bottom) incubated with increasing concentrations of NMN or M1 for 30 min. Each bar represents mean ± SD, n = 3. (E) Cryo-EM map of SARM1 2Cmut in complex with M1. The model is fitted in the map. (F) Comparison of structures of SARM1 WT (colored in yellow) and 2Cmut (colored by domains) in complex with M1. (G) Close-up view of the secondary binding interface. A portion of the BB loop (e.g., D605 and E604) in the structure of 2Cmut is resolved through interactions with the ARM domain (e.g., K173). (H) EM density of the disulfide bond in the 2Cmut at a contour level of 4σ.

Article Snippet: Similarly, 0.1 μg/μL SARM1 (WT) was incubated with 500 μM DSARM (MCE) and 500 μM NAD+ (MCE) in DPBS buffer containing an additional 150 mM NaCl and rotated at room temperature overnight.

Techniques: Activation Assay, Plasmid Preparation, Expressing, Purification, Incubation, Cryo-EM Sample Prep, Comparison, Binding Assay